Dr Sara Rollinson PhD
Research Fellow
- Email: Sara.Rollinson@manchester.ac.uk
- Telephone: +44 (0)161 275 1818
Clinical Neurosciences
Room 1.528
Stopford Building
Oxford Rd
The University of Manchester
Manchester
M13 9PT
Research
My interests inlude the genetic factors influencing susceptibility to the dementias, general genetic analysis and the statistical applications of analysis to genetic factors.
Methodological Knowledge
- Nucleic acid extraction (both of DNA and RNA)
- PCR:- standard PCR protocols plus long distance PCR, RT-PCR and differential display
- Single cell amplification of RNA
- Real time quantitative PCR using the Applied Biosystems Taqman methodology
- PCR primer design and design of Allelic discrimination assays
- Genotyping protocols: restriction fragment length polymorphism, allele specific PCR, and Taqman Allelic discrimination, Sequenom genotyping
- Genescan and sequence analysis
- Southern and northern blotting
- Cloning and manipulation of cDNA and PCR products
- Standard and differential library screening on both E.coli and Lambda based cDNA libraries
- Western blotting and ELISA
- Culture of cells/transfections
- Statistical analysis of Genotyping studies
- A wide range of bioinformatic skills, including relational database design, programming in both C and Perl, dynamic programming and analysis using Matlab, sequence and microarray analysis, and protein structure/function prediction
Publications
2009
- Rollinson, SJ, Rizzu, P, Sikkink, SSK, Baker, M, Halliwell, N, Snowden, JS, Traynor, B, Ruano, D, Cairns, N, Rohrer, J, Mead, S, Collinge, J, Rossor, M, Akay, E, Guerreiro, R, Rademakers, R, Morrison, K, Pastor, P, Alonso, E, Martinez-Lage, P, Graff-Radford, N, Neary, D, Heutink, P, Mann, DMA, Van Swieten, J, Pickering-Brown, S. (2009). Ubiquitin associated protein 1 is a risk factor for frontotemporal lobar degeneration. Neurobiol Aging, eScholarID:1d18718 | DOI:10.1016/j.neurobiolaging.2009.01.009