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School of Medicine

Professor Ann Canfield BSc, PhD

Photograph of Ann Canfield

Professor of Vascular Cell Biology

Wellcome Trust Centre for Cell-Matrix Research &  Cardiovascular Research Group
Michael Smith Building
Oxford Road
Manchester
M13 9PT

Further information can be found on the Wellcome Trust Centre for Cell-Matrix Research website.

 

Role

Ann runs a busy research lab which focusses on elucidating the molecular and cellular mechanisms underpinning vascular calcification, angiogenesis and the endothelialisation of vascular grafts. Her research is currently supported by grants from the British Heart Foundation, Wellcome Trust, BBSRC and the North-West Development Agency. She has published over 50 scientific papers and has received many invitations to present her work at National and International conferences. She has trained 12 PhD students, 5 Masters students and 1 MD student and has examined over 25 PhD and Masters theses. Ann is a member of the Biomolecular Medicine & Therapeutics Panel, Science Foundation Ireland, and was an elected committee member for the British Society of Matrix Biology with a responsibility for bursaries (2007 - 2010). She reviews manuscripts and grants for several International journals and funding agencies, respectively. Ann has been Director of the Medical Biochemistry Degree Programme since its inception in 2001 and she teaches on several undergraduate courses run by the Faculties of Life Sciences and Human & Medical Sciences.

 

Memberships of Committees and Professional Bodies

  • Member of the Biomolecular Medicine & Therapeutics Panel of the Research Frontiers Programme, Science Foundation Ireland
  • British Society of Matrix Biology
  • British Atherosclerosis Society

Research

Ann's group is at the forefront of research on the role that pericytes play in the pathophysiological process of vascular calcification. Her group was the first to demonstrate that vascular pericytes can differentiate along several lineages including osteogenic, chondrogenic and adipogenic, suggesting that these cells may represent a source of stem cells in the adult. However, when their differentiation is not properly controlled, it can lead to pathological calcification which is highly correlated with increased morbidity and mortality in patients with atherosclerosis, diabetes and end-stage renal disease and is a major cause of failure of vein grafts and replacement heart valves. She has recently demonstrated that the Wnt- and TGFbeta-signaling pathways are master controllers of pericyte differentiation and that they also contribute to the pathogenesis of vascular calcification. In addition, she has also discovered novel inhibitors of vascular calcification, Axl and its ligand, Gas6 and HtrA1 (a serine protease with the ability to regulate TGFbeta signalling). Recent evidence suggests that extensive cross-talk also exists between Axl, HtrA1, Wnt- and other growth factor receptor signaling pathways. Therefore, current research aims to determine the mechanisms by which these signaling pathways regulate pericyte differentiation and vascular calcification. Ultimately, these studies should enable us to develop approaches to (a) prevent pathological calcification, and (b) manipulate pericytes along specific lineages for therapeutic tissue regeneration.


The recruitment of pericytes to nascent endothelial cell tubes during angiogenesis is also of critical importance for the formation of patent new blood vessels. Therefore, the roles of pericyte signaling in mediating endothelial-pericyte interactions are also being investigated, using in vitro model systems developed in her laboratory. Finally, procedures to generate small diameter vascular grafts with a non-thrombogenic coating of endothelial cells are also being developed in her laboratory as part of the UK Centre for Tissue Regeneration, in collaboration with Professor Cay Kielty. They have recently demonstrated the potential of a novel hyaluronan-based biomaterial, Hyaff-11, as a scaffold for endothelial cells in tissue engineered vascular grafts. In addition, they have discovered that type VIII collagen has the potential to improve both initial cell attachment and retention of endothelial cells on vascular grafts both in vitro and in vivo. This discovery opens new avenues of research in the development of both single-stage endothelialised prostheses and the next generation of tissue engineered vascular grafts.

 

Methodological Knowledge

  • Analysis of vascular calcification
  • Isolation, culture & analysis of primary vascular cells (endothelial cells, pericytes, smooth muscle cells); culture of established cell lines
  • In vitro differentiation models (osteogenic, chondrogenic and adipogenic)
  • 3-D and 2-D cell culture models; general cell biology assays (apoptosis, adhesion, signalling etc)
  • Modulation of gene expression using recombinant adenoviruses
  • Production & purification of recombinant proteins
 

Teaching

Undergraduate:

Director of the Medical Biochemistry Degree Programme

Lecturer, Biochemical Basis of Disease (BIOL30142)

PBL Tutor, Cardiorespiratory Fitness, MBChB Degree

Post-graduate:

Ann has supervised 3 MRes, 2 MSc, 1 MD and 12 PhD students to completion. She currently supervises 2 PhD students:

Smeeta Sinha ('In vitro modelling of calcification: the effects of nitrogen and non-nitrogen containing bisphosphonates on vascular smooth muscle cells')

Lesley Anne Turner (with Prof Sandra Downes & Dr Ian Kinlock,  Materials Science) ('Nanoscaled polymer film surfaces for differentiated cell growth')

Ann would welcome enquiries from students wishing to study for a higher research degree in the general area of vascular cell biology. For further information, see: Postgraduate opportunities.

 

Biography

Ann is a member of the Cardiovascular Research Group, the Wellcome Trust Centre for Cell-Matrix Research (WTCCMR) and the UK Centre for Tissue Regeneration at the University of Manchester.

She graduated with a degree in Biochemistry from Manchester and then completed a PhD under the supervision of Professor Michael Grant at the University. Her post-doctoral studies took her to the Paterson Institute for Cancer Research. She was then awarded a Wellcome Trust Junior Research Fellowship and joined the Wellcome Trust Centre for Cell-Matrix Research in 1993. Ann was appointed a Lecturer in the Faculty of Medical and Human Sciences in 1996, Senior Lecturer in 2000 and Reader in 2007.

 

Qualifications

  • BSc (Hons), Biochemistry, 1st Class, University of Manchester
  • PhD, University of Manchester
 

Collaborators and affiliated staff

  • Dr Yvonne Alexander, Cardiovascular Research group
  • Dr Keith Brennan, WTCCMR
  • Professor Tony Day, WTCCMR
  • Dr Andrew Gilmore, WTCCMR
  • Professor Cay Kielty, WTCCMR
  • Dr Gillian Wallis, WTCCMR
  • Professor J E Davies, University of Toronto, Canada
  • Dr Sarah George, University of Bristol
 

Publications

2011

  • Liu Y, Wang T, Yan J, Heideman DA, Canfield AE, Alexander MY. (2011). HGF/c-Met signalling promotes Notch3 activation and human vascular smooth muscle cell osteogenic differentiation in vitro. Atherosclerosis, 219(2), 440-447. eScholarID:149183 | DOI:10.1016
  • Siddals KW, Allen J, Sinha S, Canfield AE, Kalra PA, Gibson JM. (2011). Apposite insulin-like growth factor (IGF) glycosylation is critical to the maintenance of vascular smooth muscle phenotype in the presence of factors promoting osteogenic differentiation and mineralization. Journal of Biological Chemistry, 286(19), 16623-16630. eScholarID:149169 | DOI:10.1074/jbc.M110.202929 jbc.M110.202929

2009

  • Alam, M, Kirton, J, Wilkinson, F, Towers, E, Sinha, S, Rouhi, M, Vizard, T, Sage, A, Martin, D, Ward, D, Alexander, MY, Riccardi, D, Canfield, AE. (2009). Calcification is associated with loss of functional calcium-sensing receptor in vascular smooth muscle cells. Cardiovasc Res, 81( 2), 260-8. eScholarID:1d21339 | DOI:10.1093/cvr/cvn279

2008

  • Hadfield K, Rock C, Inkson C, Dallas S, Sudre L, Wallis GA, Boot-Handford RP, Canfield AE. (2008). HtrA1 inhibits mineral deposition by osteoblasts: requirement for the protease and PDZ domains. J Biol Chem, 283(9), 5928-5938. eScholarID:1d21654 | DOI:10.1074/jbc.M709299200
  • Hadfield KD, Rock CF, Inkson CA, Dallas SL, Sudre L, Wallis GA, Boot-Handford RP, Canfield AE. (2008). HtrA1 inhibits mineral deposition by osteoblasts: requirement for the protease and PDZ domains. The Journal of biological chemistry, 283(9), 5928-38. eScholarID:1c7522 | PMID:18156628 | DOI:10.1074/jbc.M709299200
  • Turner N, Jones H, Davies J, Canfield AE. (2008). Cyclic stretch-induced TGFbeta1/Smad signaling inhibits adipogenesis in umbilical cord progenitor cells. Biochem Biophys Res Commun, 377( 4), 1147-51. eScholarID:1d21619 | DOI:10.1016/j.bbrc.2008.10.131

2007

  • Canfield AE, Hadfield K, Rock C, Wylie E, Wilkinson F. (2007). HtrA1: a novel regulator of physiological and pathological matrix mineralization? Biochem Soc Trans, 35( Pt 4), 669-71. eScholarID:1d21648 | DOI:10.1042/BST0350669
  • Canfield AE, Hadfield KD, Rock CF, Wylie EC, Wilkinson FL. (2007). HtrA1: a novel regulator of physiological and pathological matrix mineralisation?. Biochem Soc Trans, 35, 669-671. eScholarID:1c6970
  • Collett GDM, Sage AP, Alexander MY, Gilmore AP, Canfield AE. (2007). Axl/phosphatidylinositol 3-kinase signalling inhibits mineral deposition by vascular smooth muscle cells. Circulation Research, 100, 502-509. eScholarID:1d27417 | DOI:10.1161/01.RES.0000258854.03388.02
  • Collett GD, Sage AP, Kirton JP, Alexander MY, Gilmore AP, Canfield AE. (2007). Axl/phosphatidylinositol 3-kinase signaling inhibits mineral deposition by vascular smooth muscle cells. Circulation research, 100(4), eScholarID:1c6401 | PMID:17255529 | DOI:10.1161/01.RES.0000258854.03388.02
  • Kirton J, Crofts N, George S, Brennan K, Canfield AE. (2007). Wnt/beta-catenin signaling stimulates chondrogenic and inhibits adipogenic differentiation of pericytes: potential relevance to vascular disease? Circ Res, 101( 6), 581-9. eScholarID:1d21650 | DOI:10.1161/CIRCRESAHA.107.156372
  • Kirton JP, Crofts NJ, George SJ, Brennan K, Canfield AE. (2007). Wnt/{beta}-Catenin Signaling Stimulates Chondrogenic and Inhibits Adipogenic Differentiation of Pericytes. Potential Relevance to Vascular Disease? Circulation Research, 101, 581-589. eScholarID:1c6890 | PMID:17673669 | DOI:10.1161/CIRCRESAHA.107.156372
  • Liu Y, Wilkinson FL, Kirton JP, Jeziorska M, Iizasa H, Sai Y, Nakashima E, Heagerty AM, Canfield AE, Alexander MY. (2007). Hepatocyte growth factor and c-Met expression in pericytes: implications for atherosclerotic plaque development. J Pathol, 212(1), 12-19. eScholarID:1c6971
  • Liu YY, Wilkinson FL, Kirton J, Jeziorska M, Iizasa H, Sai Y, Nakashima E, Heagerty AM, Canfield AE, Alexander MY. (2007). Hepatocyte Growth Factor and c-Met Expression in Pericytes: Implications for atherosclerotic plaque development. Journal of Pathology, 212(1), 12-19. eScholarID:1d27332 | DOI:10.1002/path.2155
  • Wilkinson FL, Liu YY, Rucka A, Jeziorska M, Hoyland JA, Heagerty AM, Canfield AE, Alexander MY. (2007). Contribution of VCAF-positive cells to neovacularisation and calcification in atherosclerotic plaque development. Journal of Pathology, 211(3), 362-369. eScholarID:1d27330 | DOI:10.1002/path.2114
  • Williamson M, Shuttleworth A, Canfield AE, Black R, Kielty C. (2007). The role of endothelial cell attachment to elastic fibre molecules in the enhancement of monolayer formation and retention, and the inhibition of smooth muscle cell recruitment. Biomaterials, 28( 35), 5307-18. eScholarID:1d21652 | DOI:10.1016/j.biomaterials.2007.08.019
  • Williamson MR, Shuttleworth CA, Canfield AE, Black RA, Kielty CM. (2007). The role of endothelial cell attachment to elastic fibre molecules in the enhancement of monolayer formation and retention, and the inhibition of smooth muscle cell recruitment. Biomaterials, 28, 5307-5318. eScholarID:1c6972

2006

  • Kirton JP, Wilkinson FL, Canfield AE & Alexander MY. (2006). Dexamethasone down-regulates calcification-inhibitor molecules and accelerates osteogenic differentiation of vascular pericytes: implications for vascular calcification. Circulation Research, 98, 1264-1272. eScholarID:1c6309 | DOI:10.1161/01.RES.0000223056.68892.8b
  • Kirton JP, Wilkinson FL, Canfield AE, M. Yvonne Alexander. (2006). Dexamethasone down-regulates calcification-inhibitor molecules and accelerates osteogenic differentiation of vascular pericytes; implications for vascular calcification. Circulation Research, 98, 1264-1272. eScholarID:1d26761 | DOI:10.1161/01.RES.0000223056.68892.8b
  • Turner Neill J, Michael O Murphy, Kielty CM, C Adrian Shuttleworth, Black RA, Martin J Humphries, Walker MG, Canfield AE. (2006). alpha2(VIII) collagen substrata enhance endothelial cell retention under acute shear stress flow via an alpha2beta1 integrin dependent mechanism: an in vitro and in vivo study. Circulation, 114, 820-829. eScholarID:1d9023 | DOI:10.1161/CIRCULATIONAHA.106.635292
  • Turner NJ, Murphy MO, Kielty CM, Shuttleworth CA, Black RA, Humphries MJ, Walker MG, Canfield AE. (2006). Alpha2(VIII) collagen substrata enhance endothelial cell retention under acute shear stress flow via an alpha2beta1 integrin-dependent mechanism: an in vitro and in vivo study. Circulation, 114(8), 820-829. eScholarID:1c5344 | PMID:16908762 | DOI:10.1161/CIRCULATIONAHA.106.635292

2005

  • Alexander MY, Wilkinson FL, Kirton J, Rock C, Collett GGDM, Jeziorska M, Smyth J, Heagerty AM, Canfield AE. (2005). Identification and characterization of vascular calcification-associated factor, a novel gene upregulated during vascular calcification in vitro and in vivo. Arterioscler Thromb Vasc Biol, 25(9), eScholarID:1d12061
  • Alexander MY, Wilkinson FL, Kirton JP, Rock CF, Collett GD, Jeziorska M, Smyth JV, Heagerty AM, Canfield AE. (2005). Identification and characterization of vascular calcification-associated factor, a novel gene upregulated during vascular calcification in vitro and in vivo. Arteriosclerosis, thrombosis, and vascular biology, 25(9), 1851-1857. eScholarID:1c3945 | DOI:10.1161/01.ATV.0000175750.94742.46
  • Collett G, Canfield AE. (2005). Angiogenesis and pericytes in the initiation of ectopic calcification. Circ Res, 96( 9), 930-8. eScholarID:1d21657 | DOI:10.1161/01.RES.0000163634.51301.0d
  • Collett GDM, Canfield AE. (2005). Angiogenesis and pericytes in the initiation of ectopic calcification. Circulation research, 96, 930-938. eScholarID:1c3819

2004

  • Hardingham TE, Kielty CM, Canfield AE, TEW SR, Ball SS, Turner NJ, Ratcliffe KE. (2004). Cell culture in tissue engineering. Biological Medicines from Cell Culture. John Wiley. eScholarID:3d365
  • Wilkinson FW, Kirton JP, Canfield AE, Alexander MY. (2004). The expression of a novel gene C15, is modulated by inflammatory agents in vascular cells. eScholarID:2d1698
  • Day J, Olin A, Murdoch A, Canfield AE, Sasaki T, Timpl R, Hardingham T, Aspberg A. (2004). Alternative splicing in the aggrecan G3 domain influences binding interactions with tenascin-C and other extracellular matrix proteins. J Biol Chem, 279( 13), 12511-8. eScholarID:1d21645 | DOI:10.1074/jbc.M400242200
  • Day JM, Olin AI, Murdoch AD, Canfield A, Sasaki T, Timpl R, Hardingham TE, Aspberg A. (2004). Alternative splicing in the aggrecan G3 domain influences binding interactions with tenascin-C and other extracellular matrix proteins. Journal of Biological Chemistry, 279(13), 12511-8. eScholarID:1c2205 | PMID:14722076 | DOI:10.1074/jbc.M400242200
  • Farrington-Rock C, Crofts NJ, Doherty MJ, Ashton BA, Griffin-Jones C, Canfield AE. (2004). Chondrogenic and adipogenic potential of microvascular pericytes. Circulation, 110, 2226-2232. eScholarID:1c2639 | DOI:10.1161/01.CIR.0000144457.55518.E5
  • Farrington-Rock C, Crofts NJ, Doherty MJ, Ashton BA, Griffin-Jones C, Canfield AE. (2004). Chondrogenic and adipogenic potential of microvascular pericytes. Circulation, 110, 2226-2232. eScholarID:1d25969 | DOI:10.1161/01.CIR.0000144457.55518.E5
  • Turner N, Kielty C, Walker M, Canfield AE. (2004). A novel hyaluronan-based biomaterial (Hyaff-11) as a scaffold for endothelial cells in tissue engineered vascular grafts. Biomaterials, 25( 28), 5955-64. eScholarID:1d21658 | DOI:10.1016/j.biomaterials.2004.02.002
  • Turner NJ, Kielty CM, Walker MG, Canfield AE. (2004). A novel hyaluronan biomaterial (Hyaff-11)as a scaffold for endothelial cells in tissue engineered vascular grafts. Biomaterials, 25, 5955-5964. eScholarID:1c2273 | DOI:10.1016/j.biomaterials.2004.02.002

2003

  • Collett G, Wood A, Alexander MY, Varnum BC, Boot-Handford RP, Ohanian V, Ohanian J, Fridell YW, Canfield AE. (2003). Receptor tyrosine kinase Axl regulates the osteogenic differentiation of pericytes. Circulation research, 92, 1123-1129. eScholarID:1c2549 | DOI:10.1161/01.RES.0000074881.56564.46
  • Collett G, Wood A, Alexander MY, Varnum BC, Boot-Handford RR, Ohanian V, Ohanian J, Fridell YW, Canfield AE. (2003). Receptor tyrosine kinase Axl modulates the osteogenic differentiation of pericytes. Circulation Research, 92, 1123-1129. eScholarID:1d25185 | DOI:10.1161/01.RES.0000074881.56564.46

2002

  • Canfield AE, Farrington C, Dziobon MD, Boot-Handford RP, Heagerty AM, Kumar SN, Roberts ISD. (2002). The involvement of matrix glycoproteins in vascular calcification and fibrosis: an immunohistochemical study. J Path 196, 228-234. Journal of Pathology, 196, 228-234. eScholarID:1c2552 | DOI:10.1002/path.1020
  • Canfield AE, Farrington Clare, Dziobon M, Boot-Handford RR, Heagerty AM, Kumar Shant, Roberts Ian S. (2002). The involvement of matrix glycoproteins in vascular calcification and fibrosis: an immunohistochemical study. J Pathol, 196( 2), 228-34. eScholarID:1d19383 | DOI:10.1002/path.1020
  • Flasza M, Gorman P, Roylance R, Canfield AE, Baron M. (2002). Alternative splicing determines the domain structure of WWP1, a Nedd4 family protein. Biochem Biophys Res Commun 290, 431-437. Biochemical and Biophysical Research Communication, 290, 431-437. eScholarID:1c2551 | DOI:10.1006/bbrc.2001.6206
  • Flasza M, Gorman P, Roylance R, Canfield AE, Baron M. (2002). Alternative splicing determines the domain structure of WWP1, a Nedd4 family protein. Biochem Biophys Res Commun, 290( 1), 431-7. eScholarID:1d21660 | DOI:10.1006/bbrc.2001.6206
  • Flasza M, Gorman P, Roylance R, Canfield AE, Baron M. (2002). Alternative splicing determines the domain structure of WWP1, a Nedd4 family protein. Biochemical and biophysical research communications, 290(1), 431-7. eScholarID:1c1390 | PMID:11779188 | DOI:10.1006/bbrc.2001.6206

2001

  • Sinha SS, Kielty CM, Heagerty AM, Canfield AE, Shuttleworth CC.A. (2001). Upregulation of collagen VIII following porcine coronary artery angioplasty is related to smooth muscle cell migration not angiogenesis. Int J Exp Pathol, 82( 5), 295-302. eScholarID:1d19385

2000

  • Canfield AE, Doherty M, Kelly V, Newman B, Farrington C, Grant M, Boot-Handford R. (2000). Matrix Gla protein is differentially expressed during the deposition of a calcified matrix by vascular pericytes. FEBS Lett, 487( 2), 267-71. eScholarID:1d21661
  • Canfield AE, Doherty MM.J, Wood AA.C, Farrington CC, Begum N, Harvey B, Poole A, Grant MM.E, Boot-Handford RR. (2000). Role of pericytes in vascular caclification: a review. Z. Kardiol, 89.2, 20-27. eScholarID:1d25186
  • Canfield, AE., Doherty, M, Wood, A, Farrington, C, Ashton, B, Begum, N, Harvey, B, Poole, A, Grant, M, Boot-Handford, R. (2000). Role of pericytes in vascular calcification: a review. Z Kardiol, 89 Suppl 2, 20-7. eScholarID:1d21669
  • Green H, Canfield AE, Hillarby M, Grant M, Boot-Handford RR, Freemont AJ, Wallis GA. (2000). The ribosomal protein QM is expressed differentially during vertebrate endochondral bone development. J Bone Miner Res, 15( 6), 1066-75. eScholarID:1d9645
  • H Green, Canfield AE, Hillarby M, M E Grant, Boot-Handford RR, Freemont AJ, Wallis Gillian. (2000). The ribosomal protein "QM" is differentially expressed during vertebrate endochondral bone development. Journal of Bone Mineral Research, 15, eScholarID:1d23802
  • Mantell D, Owens P, Bundred N, Mawer E, Canfield AE. (2000). 1 alpha,25-dihydroxyvitamin D(3) inhibits angiogenesis in vitro and in vivo. Circ Res, 87( 3), 214-20. eScholarID:1d21664

1999

  • Doherty M, Canfield AE. (1999). Gene expression during vascular pericyte differentiation. Crit Rev Eukaryot Gene Expr, 9( 1), 1-17. eScholarID:1d21671
  • Doherty MM.J, Canfield AE. (1999). Gene expression during vascular pericyte differentiation. Critical Reviews in Eukaryotic Gene Expression, 9.1, 1-17. eScholarID:1d23735
  • Canfield AE, AC Wood, MJ Doherty, Grant MM.E, Boot-Handford RR. (1999). Identification and functional analysis of genes involved in the osteogenic differentiation of pericytes. Journal of Bone Mineral Research, 14, P27. eScholarID:17d814
  • Harvey B, Wood AC, Boot-Handford RP, Canfield AE. (1999). Characterisation of genes down-regulated during the depostion of a mineralised matrix by pericytes in vitro. Journal of Bone Mineral Research, 12, P5+1. eScholarID:17d202

1998

  • Doherty M, Ashton B, Walsh S, Beresford J, Grant M, Canfield AE. (1998). Vascular pericytes express osteogenic potential in vitro and in vivo. J Bone Miner Res, 13( 5), 828-38. eScholarID:1d21672
  • Doherty M, Boot-Handford R, Grant M, Canfield AE. (1998). Identification of genes expressed during the osteogenic differentiation of vascular pericytes in vitro. Biochem Soc Trans, 26( 1), eScholarID:1d21666
  • Farrington CC, Roberts I, Heagerty AM, Canfield AE. (1998). The expression of cartilage oligomeric matrix protein, thrombospondin-1, bone sialoprotein and osteopontin in calcified and non-calcified arterial lesions. Biochem Soc Trans, 26( 1), eScholarID:1d19389
  • Canfield AE. (1998). Vascular pericytes - the link between angiogenesis and osteogenesis. Journal of Vascular Research, 35, 373. eScholarID:17d101
  • DJ Mantell, Mawer BB, NJ Bundred, Canfield AE. (1998). The effects of 1,25-dihydroxyvitamin D-3 on angiogenesis in vitro. Journal of Vascular Research, 35, 390. eScholarID:17d247
  • M Kale, Canfield AE. (1998). Expression of ribosomal proteins during the early stages of VEGF-induced angiogenesis. Journal of Vascular Research, 35, 389-390. eScholarID:17d134
  • MJ Doherty, Boot-Handford RR, Grant MM.E, Canfield AE. (1998). Identification of genes expressed during the osteogenic differentiation of vascular pericytes in vitro. Biochem. Soc. Trans, 26, S4. eScholarID:17d246
  • Wood A, Harvey B, Neild H, Doherty M, Boot-Handford RR, Canfield AE. (1998). Identification of genes which are down-regulated during the osteogenic differentiation of pericyte. Journal of Vascular Research, 35, 391. eScholarID:17d248

1997

  • Grant MM.E, MJ Doherty, BA Ashton, S Walsh, JN Beresford, Canfield AE. (1997). Osteogenic differentiation of vascular pericytes in vitro and in vivo. eScholarID:2d1020
  • ISD Roberts, Farrington CC, DJA Goldsmith, HS Neild, McCollum CN, Heagerty AM, Canfield AE. (1997). The expression of osteopontin and thrombospondin-1 in calcified and non-calcified arterial lesions. Journal Of Pathology, 181, A31. eScholarID:17d99
  • ISD Roberts, Farrington CC, M Dziobon, Brenchley PEC, Short CC, Canfield AE. (1997). Thrombospondin-1 expression in renal allograft rejection. Journal Of Pathology, 181, A53. eScholarID:17d588
  • MD Dziobon, Canfield AE, ISD Roberts. (1997). Expression of osteopontin in renal allograft rejection. Journal Of Pathology, 182, A36. eScholarID:17d391

1996

  • Canfield AE, Sutton A, Hoyland J, Schor A. (1996). Association of thrombospondin-1 with osteogenic differentiation of retinal pericytes in vitro. J Cell Sci, 109 ( Pt 2), 343-53. eScholarID:1d21674
  • MJ Doherty, BA Ashton, Grant MM.E, Canfield AE. (1996). Expression of osteogenic markers by vascular pericytes. Journal of Bone Mineral Research, 11, eScholarID:1d24932
  • H Nield, MJ Doherty, Boot-Handford RR, Canfield AE. (1996). The involvement of vascular pericytes in arterial calcification. Journal of Vascular Research, 33 Supp 1, 73. eScholarID:17d133
  • MJ Doherty, BA Ashton, Grant MM.E, Canfield AE. (1996). Vascular pericytes express osteogenic markers in vitro and form bone in vivo. International Journal Of Experimental Pathology, 77, A4. eScholarID:17d245

1995

  • Canfield AE, Schor A. (1995). Evidence that tenascin and thrombospondin-1 modulate sprouting of endothelial cells. J Cell Sci, 108 ( Pt 2), 797-809. eScholarID:1d21676
  • DRR Hiscock, Canfield AE, JT Gallagher. (1995). Molecular structure of heparan sulphate synthesised by bovine aortic endothelial cells. Biochim. Biophys. Acta, 1244, 104-112. eScholarID:1d24930
  • Schor A, Canfield AE, Sutton A, Arciniegas E, Allen T. (1995). Pericyte differentiation. Clin Orthop Relat Res, ( 313), 81-91. eScholarID:1d21675
  • Schor A, Canfield AE, Sutton A, Arciniengas E, Allen T. (1995). Pericyte differentiation. Clinical Orthopaedics And Related Research, 31`3, 81-91. eScholarID:1d24929
  • MJ Doherty, BA Ashton, Grant MM.E, Canfield AE. (1995). Vascular pericytes as possible osteoprogenitor cells. Bone, 17, 562. eScholarID:17d587

1994

  • Canfield AE, Schor A. (1994). Heterogeneity in collagen biosynthesis by sprouting retinal endothelial cells. J Cell Physiol, 159( 1), 19-28. eScholarID:1d21678 | DOI:10.1002/jcp.1041590104
  • Canfield AE, Sutton A, Hiscock D, Gallagher J, Schor A. (1994). Alpha- and beta-xylosides modulate the synthesis of fibronectin and thrombospondin-1 by endothelial cells. Biochim Biophys Acta, 1200( 3), 249-58. eScholarID:1d21677
  • Canfield AE, Sutton A, Schor A. (1994). Association of thrombospondin gene expression with osteogenic differentiation of vascular pericytes. International Journal Of Experimental Pathology, 75, A76-A77. eScholarID:17d38

1993

  • AM Schor, Canfield AE. (1993). Role of the extracellular matrix in angiogenesis: xylosides inhibit matrix biosynthesis and sprouting in endothelial cell cultures. Eur. Resp. Rev, 3, 644-649. eScholarID:1d24928

1992

  • Canfield AE, Sutton A, Schor S, Grant M, Schor A. (1992). Aortic endothelial cells in culture: factors which modulate their morphological and biosynthetic phenotypes. EXS, 61, 158-66. eScholarID:1d21681
  • Canfield AE, Wren F, Schor S, Grant M, Schor A. (1992). Aortic endothelial cell heterogeneity in vitro. Lack of association between morphological phenotype and collagen biosynthesis. J Cell Sci, 102 ( Pt 4), 807-14. eScholarID:1d21679
  • Schor A, Canfield AE, Sutton A, Allen T, Sloan P, Schor S. (1992). The behaviour of pericytes in vitro: relevance to angiogenesis and differentiation. EXS, 61, 167-78. eScholarID:1d21680

1991

  • Canfield AE, Schor A. (1991). Identification and partial characterisation of a low Mr collagen synthesised by bovine retinal pericytes. Apparent relationship to type X collagen. FEBS Lett, 286( 1-2), 171-5. eScholarID:1d21685
  • Murdoch A, Canfield AE, Grant M, Boot-Handford R. (1991). Maintenance of collagen IV expression by bovine retinal pericytes isolated directly onto Matrigel film. Biochem Soc Trans, 19( 4), eScholarID:1d21682
  • Schor A, Canfield AE, Sloan P, Schor S. (1991). Differentiation of pericytes in culture is accompanied by changes in the extracellular matrix. In Vitro Cell Dev Biol, 27A( 8), 651-9. eScholarID:1d21684
  • Sutton A, Canfield AE, Schor S, Grant M, Schor A. (1991). The response of endothelial cells to TGF beta-1 is dependent upon cell shape, proliferative state and the nature of the substratum. J Cell Sci, 99 ( Pt 4), 777-87. eScholarID:1d21683

1990

  • Canfield AE, Allen T, Grant M, Schor S, Schor A. (1990). Modulation of extracellular matrix biosynthesis by bovine retinal pericytes in vitro: effects of the substratum and cell density. J Cell Sci, 96 ( Pt 1), 159-69. eScholarID:1d21688
  • Canfield AE, Boot-Handford R, Schor A. (1990). Thrombospondin gene expression by endothelial cells in culture is modulated by cell proliferation, cell shape and the substratum. Biochem J, 268( 1), 225-30. eScholarID:1d21687
  • Schor A, Allen T, Canfield AE, Sloan P, Schor S. (1990). Pericytes derived from the retinal microvasculature undergo calcification in vitro. J Cell Sci, 97 ( Pt 3), 449-61. eScholarID:1d21686

1989

  • Canfield AE, AM Schor, DJ Loskutoff, SL Schor, Grant MM.E. (1989). Plasminogen activator inhibitor-type I is a major biosynthetic product of retinal microvascular endothelial cells and pericytes in culture. Biochemical Journal, 259, 529-535. eScholarID:1d24921
  • Canfield AE, Boot-Handford RR, AM Schor. (1989). Thrombospondin gene expression by endothelial cells in culture is modulated by cell proliferation, cell shape and the substratum. Biochemical Journal, 268, 225-230. eScholarID:1d24922
  • Canfield AE, Schor A, Loskutoff D, Schor S, Grant M. (1989). Plasminogen activator inhibitor-type I is a major biosynthetic product of retinal microvascular endothelial cells and pericytes in culture. Biochem J, 259( 2), 529-35. eScholarID:1d21689

1987

  • Canfield AE, AM Schor, DC West, SL Schor, Grant MM.E. (1987). Identification and partial characterisation of two major proteins of Mr47000 synthesised by bovine retinal endothelial cells in culture. Biochemical Journal, 246, 121-129. eScholarID:1d24920
  • Canfield AE, Schor A, West D, Schor S, Grant M. (1987). Identification and partial characterization of two major proteins of Mr 47,000 synthesized by bovine retinal endothelial cells in culture. Biochem J, 246( 1), 121-9. eScholarID:1d21690

1986

  • Canfield AE, AM Schor, SL Schor, Grant MM.E. (1986). The biosynthesis of extracellular matrix components by bovine retinal endothelial cells displaying distinctive morphological phenotypes. Biochemical Journal, 235, 375-383. eScholarID:1d24919
  • Canfield AE, Schor A, Schor S, Grant M. (1986). The biosynthesis of extracellular-matrix components by bovine retinal endothelial cells displaying distinctive morphological phenotypes. Biochem J, 235( 2), 375-83. eScholarID:1d21691

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